Nanobrook 173 Omni

Features at a glance

SIZING

  • Rapid and accurate nanoparticle size distributions
  • Multimodal & unimodal size distribution software
  • ISO 13321 and ISO 22412 compliant results
  • Range: < 0.3 nm to 10 µm
  • Three measurement angles: 15°, 90°, and 173°
  • Ideal for fast, routine sizing applications in research or quality control
  • High power 35 mW diode laser
  • Dynamic light scattering at 173° and 90°
  • Temperature control, -5 °C to 110 °C
  • Compact bench top unit, USB connection
  • Molecular weight determination (relative and absolute through Debye plot)
     

ZETA POTENTIAL

  • Zeta potential for the difficult cases...
  • For proteins, peptides, mAb, RNA and other biological samples
  • For zeta potential in organic solvents
  • For oily or viscous media
  • For high-salt suspensions
  • For samples near the I.E.P.
  • 1,000 times more sensitive than other techniques
  • Disposable cuvettes, no contamination or alignment
  • Built in automatic procedures and parameters (SOP)
  • Easy to use
     

Brookhaven's NanoBrook Omni instrument combines the best technology from our particle/protein sizer and zeta potential analyzer in to one powerful and accurate solution for sizing and zeta potential analysis. Measure samples in nearly every possible suspension environment from high salts such as PBS and sea water to organic solvents and of course aqueous solutions. Particle and protein sizing, with the highest sensitivity, utilizing three measurement angles for truly unbiased measurement results. The NanoBrook Omni utilizes a backscattering angle as well as 90° and 15°.

The NanoBrook Omni is the one true “All-in-One” solution to particle and protein characterization.

Rapid, Reliable, and Accurate Analysis

The new NanoBrook Omni particle size and zeta potential analyzer incorporates all you need for fast, routine, sub-micron measurements of size and zeta potential. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, and based on doppler velocimetry (electrophoretic light scattering, ELS) for zeta potential measurement, most measurements only take a minute or two. The instrument also includes Phase Analysis Light Scattering (PALS) measurements for samples with low mobilities.

Three Scattering Angles:

Measurements of traditional colloids are usually made at 90° scattering angle due to the unbiased results measured. For nanoparticles and proteins, IgG and peptides, these < 50 nm samples can be measured using the backscattering angle (173°) for best S/N and reproducibility of measurements. Finally the 15° detection angle can be selected for added sensitivity with aggregation measurements. Zeta potential measurements are always performed using the 15° detection angle to minimize diffusion broadening.

Principles of Operation - Sizing

Dilute suspensions, on the order of 0.0001 to 1.0% v/v are prepared, using suitable wetting and/or dispersing agents, if required. A small ultrasonicator is sometimes useful in breaking up loosely-held agglomerates. At 173° sample volume may be reduced to 50 µL with a polystyrene, U-shaped, disposable cuvette and the sample is recoverable. At 90° square polystyrene or glass cells (two or three mL) are used, one as small as 10 µL (non-disposable). In addition, disposable, glass round cells with reusable Teflon stoppers are used for aggressive solvent suspensions. In all case, just a few minutes are required for the sample and cell to equilibrate with the actively controlled temperature environment inside the NanoBrook Omni.

Reproducibility

Perhaps the most important benefit for users is the reproducibility from sample-to-sample, operator-to-operator, and instrument-to-instrument. The table below shows the results obtained for a polystyrene latex. Notice the Std. Error of the Effective (or Average) Diameter is less than 1% of the mean of the 10 repeated runs; the duration of each run was only five minutes. Such excellent repeatability is typical of the 90Plus for even routine QC analysis and ensures product quality time-after-time.

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