Enverdi BOD



This new product is based on the use of a bioreagent, which is chemically reduced in microbial cells during the aerobic respiration, leading to an increase in the fluorescence signal. Fluorescence intensity, recorded throughout the

incubation is directly proportional to the amount of organic substrates biodegraded.

Definite results are reached after 48 hours of incubation at 30°C.

The endpoint concentration provided by the Enverdi-BOD kit is directly expressed in mg O2/L, like for the reference method.

Ready-to-use solution

To simplify the BOD deternimation using the Enverdi method, we developped a dedicated standard bacterial seed (OptiSeed). This ready-to-use solution, including Reagents + Calibration Standards + Microplate + Bacterial Seed + Fluorescence microplate reader, allows to secure and standardize your BOD tests.


Enverdi-BOD provides the user with many features:

- Fast: analytical results obtained in 48 hours

- Profitable: the microplate format provides a sharp decrease in analytical costs, up to 3-fold

- High-throughput: one kit enables to perform up to 80 BOD5 (no replicates) or 40 (with duplicates)

- Unique: unique analytical tool for regulatory analysis and WWTPs’ operation monitoring

- Miniature: incubation takes place directly in the fluorescence reader, bench surface less than 1 m2

- Simple: 45 minutes are required to prepare 40 duplicated samples, which is 5 to 10 times faster than the conventional method

- Automated: data recording and management are fully automated
- Ready-to-use: ready-to-use solution including reagents - calibration standards - microplate - bacterial seed - fluorescence microplate reader


International validation

The international validation of the BDO5 substitute Enverdi was published in the Environmental science and pollution research international in June 2014.

The detailed results of the study runned in France and in the US show the robust equivalence of the Envolure technology (48 h) against the reference method (5 days).

To meet the growing challenges that the wastewater treatment professionals have to deal with, Envolure (AMS Alliance Group) developed the analytical solution: Enverdi -Process.

Enverdi-Process is an analytical solution based on the same technical principle as the kit: Enverdi-DBO/BOD. This technology uses a chemical probe or bioreagent which during the aerobic degradation metabolism of a bacterial biomass will be chemically reduced by the activity of the biomass and will generate a fluorescent emission.

Based on this principle, Enverdi-Process offers professionals of the waste water sector (industrial or urban), a fast and efficient tool for better control and an optimization of the treatment of their wastewater.


The Enverdi -Process kit offers the following options:

- Toxicity/Inhibition assay

- The kit can reveal in 24 hours the presence of a potential inhibitor or toxic agent in the wastewater. The operator can therefore quickly act on the treatment process. The assay is performed directly on the WWTP biomass and does not use any external reagents or biological references to avoid any biased results.


Possible applications:

- Determining the toxicity threshold of a toxic compound to the existing biomass

- Analyzing the recalcitrance of an industrial biocide

- Determining the most resistant bacterial strains to certain chemicals


Aerobic bioactivity assay

The kit can be used to monitor the level of activity of a biomass over time. Results can be gathered very quickly, usually in approximately 2 hours, and can therefore be used to act upon the treatment processes.


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